Production Of Transgenic Animals Notes

A transgenic animal is an animal in which one or more genes have been introduced into its nonreproductive cells.

Production of transgenic animals notes. The first transgenic animal was produced in 1983 when genes for human growth hormone were introduced into mice. Applications of Genetically modified animals. Transgenic animal technologies have come a long way since the creation of the first transgenic mouse in 1974 Transgenic Mouse 2005.

ES cells are obtained from the inner cell mass of a blastocyst. Transgenic animals have the potential of agricultural applications like improved growth rate and carcass composition improved resistance to disease increased milk. The cells are then incorporated into an embryo at the blastocyst stage of development.

Transgenic mice are developed by injecting DNA into the oocytes or 1-2 celled embryos taken from female mice. Microinjection blastocyst injection and using a retrovirus Nuclear transfers Artificial chromosomes for gene transfer PRODUCTION OF TRANSGENIC ANIMALS THE METHODOLOGY. The foreign gene is constructed using recombinant DNA methodology.

Milk as the Medium of Protein Production. The main principle in the production of transgenic animals is the introduction of a foreign gene or genes into an animal the inserted genes are called transgenes. Genetically modified animals are proving ever more vital in.

The transgenic expression is not only used to breed animals which have specific traits but also to breed animals that are lacking specific genes This technique is called as knockout or gene. A Gene requires certain cellular mechanism to help for the production of protein. In addition to the gene itself the DNA usually includes other sequences to enable it to be incorporated into the DNA of the host and to be expressed correctly by the cells of the host.

There are many ways to produce transgenic animals. Theses mechanism is absent in cell culture. Transgenic animals can be created by manipulating embryonic stem cells.